Our data indicates that asthma and allergic diseases are common in Singapore, with up to 20% of school children with diagnosed asthma. Morbidity of asthma is also substantial in terms of hospital admissions and the number of bed days utilised. The economic burden is considerable, amounting to S$57 million/ annum, and direct costs are equivalent to 1.2% of our healthcare bill.
Our research group is one of the earliest in defining the allergens of this region, with more than 100 international publications in the subject of allergy and immunology, several patents approved and submitted, and awards received.
We are therefore poised to face the challenges in this field of research and continue in our pursuit to achieve research excellence in the following research areas listed below.
We have cloned and fully characterised a panel of 12 allergens from Blomia tropicalis mites. Recombinant mite allergens expressed from the cDNA clones have served as useful reagents for diagnosis and can be further exploited for recombinant allergen-based immunotherapy of mite allergy related asthma and rhinitis. Allergens array, DNA immunisation-induced allergen-specific therapeutic monoclonal antibodies and component-resolved diagnostic and therapeutic reagents using recombinant mite allergens are now being developed.
Lactic acid bacteria have been shown to reduce the Th2 cytokine production from allergic peripheral blood mononuclear cells when re-stimulated with the related allergen. In addition, it can induce the production of regulatory cytokines like IL-10 and TGF-β in the mucosal tissues. These data strongly suggests that lactic acid bacteria may be useful in the treatment and prevention of allergic diseases. Animal models of allergic asthma and allergic dermatitis are used to explore the effectiveness of wild type and recombinant lactic acid bacteria for oral vaccination. The interaction of gut dendritic cells with the probiotics will be further elucidated to understand the immunomodulatory effects of probiotics on the Th2 immune responses. The long-term goal of this research project is to develop oral vaccines for allergic asthma and to study the mechanism of the mucosal vaccination.
Allergen gene vaccination approach has been demonstrated to result in an inhibition of allergen-specific IgE and Th2 response in mice. This is a novel proof-of-concept work and our data suggests that DNA vaccination is a feasible approach to prevent and control allergic asthma as well as other allergic disorders. We are now studying the mechanism of DNA vaccination, including DNA uptake by dendritic cells, regulation of Th2 immune responses and IgE production and the long-term memory immune response elicited by DNA immunisation. We will also focus our future research on the optimisation of this technology for clinical trials.
We have identified and characterised a potent immunomodulatory protein from an edible mushroom. The potential use of such molecule for cancer immunotherapy and adjuvant of viral and allergen vaccine had been proven conceptually in our laboratory. We will further consolidate the application by performing mechanistic studies related to this immunomodulatory protein.
Eczema is a common chronic inflammatory skin disorder with significant impact on child health and its prevalence is increasing worldwide. It is known that the interaction between gut microbes and the host immune system influences early life development of the immune system. We and others have shown that the composition of the stool microbiota signatures differ between eczema and healthy controls in infancy. We hypothesise that the immune dysregulation associated with childhood eczema is influenced at least in part by the products of certain gut bacterial structural and functional genes, e.g. (i) virulence genes and (ii) genes involved in carbohydrate metabolism. We aim to assess the structural and functional gene composition of stool microbiota of 10 infants with eczema and their matched (for age, gender, mode of delivery, feeding) controls at the age of 3 months. The scope of work will include the identification of functional and structural gene composition present in the stools of infants with and without eczema by comparative metagenomics using 454 pyrosequencing.
Early childhood wheezing increases the risk of subsequent asthma. Although the allergic predisposition is an important risk factor for these disorders, our recent studies also show that wheezing in early life could be due to pre-existent immune aberrations in cord blood cells. Our earlier studies have shown that cord blood cells of infants who subsequently wheeze in the first 2 years of life have abnormal heightened responses of the innate compartment of immune cells when stimulated with mitogens (chemicals) in laboratory culture. We therefore hypothesise that these immune cells of the innate immunity could be aberrant in these infants and that this abnormality would lead to an increased susceptibility to lung inflammation which manifests as wheezing disorders. We aim to study and characterise these immune abnormalities in infants with wheezing disorders. We will study in detail the responses of monocytes (specialised cells of the innate immune system) from cord blood and peripheral blood of these infants at 1 year of age and their matched healthy controls. In addition, the subsequent effects on the T helper cells (specialised cells of the adaptive immune compartment) will be studied in the blood of these infants at the age of 1 year. Using molecular techniques and specialised protein array methods, the profile of proteins (cytokines) released by the immune cells of the cord blood and (1 year old) peripheral blood, and their genes expressed will be studied and compared with healthy controls. The findings of this proposal will provide us with a better understanding of the immune signatures of wheezing infants and how they could lead to inflammatory processes in the lung. It also has the potential of discovering novel or diagnostic biomarkers in infants who are susceptible to wheeze.
Our research activities focus on establishing and developing genetic research and diagnostic facilities in cardiovascular genetics, with emphasis on mutation screening, genotyping and genetic epidemiological analyses.
In our research on genes related to Coronary Artery Disease (CAD) together with genetic epidemiological studies of how genes influence plasma risk factors and clinical outcome, it has led us to develop and patent an algorithm to predict an individual's risk of heart disease using information from genetic and environmental risk factors. The algorithm allows clinicians to predict and identify patients with high heart disease risk. Included in the suite of 32 genetic markers are markers that are known to predispose one to high blood pressure or high cholesterol. The test has up to 83% level of accuracy in predicting CAD risk and is currently licensed for commercialisation.
We also focus our cardiovascular research on characterising the functional role of an androgen-dependent TFPI regulating protein (ADTRP) and an acute phase protein called Serum Amyloid A (SAA) in the pathogenesis of CAD.
The division is also well plugged into regional and international genetic epidemiology consortia which perform meta-analyses on cardio-metabolic diseases for very large cohort of subjects.
Our team of researchers focus on clinically relevant research with the aim of improving outcomes for children with various developmental disorders. The field of clinical research in developmental and behavioural paediatrics is an emerging one in Singapore with vast potential implications for policy and practices.
Beyond descriptive research, we emphasise the need for meaningful interventions that can be employed so as to actively change the paths and outcomes for children with special needs. Our scientific work has been published in international peer reviewed journals and presented regularly at numerous local and international conferences with awards won. Our team also collaborates with national and community organisations (e.g. NCSS, MOE, MCYS) for various projects such as those that evaluate current practices in the early intervention sector and explore new models of care.
We welcome other potential collaborations from hospitals and agencies to participate in research.
Some of the broad areas of research that we are involved in include:
We have multiple ongoing studies, including those exploring new models of care for children with Autism such as:
Our researchers are involved in the GUSTO birth cohort (Growing Up in Singapore Towards healthy Outcomes), studying factors affecting child development including socio-economic status and maternal mood. This also includes examining predictors of developmental outcomes of children including cognition, executive function and school-readiness.
Studies in this area include the effects of screen time exposure on children's cognition, behaviour and social emotional development as well as implementation and evaluation of effective interventions to tackle screen time exposure. Research in this area also encompasses looking at family and environmental factors that can have a positive or negative impact on screen time exposure and its consequences.
We have a comprehensive ongoing programme studying the unmet needs of children and their families of chronic medical illnesses and the cognitive and behavioural profiles of these children. This involves examining the effects of chronic disease and its treatment on children's development among other aspects.
Through this programme, we will establish the overall burden of care in these children so as to facilitate better resource planning. This includes children who are survivors of haematological cancers and stem cell/bone marrow transplant recipients, children with congenital cardiac disease who are post cardiac surgery, children born premature, children with epilepsy or brain tumours and children with chronic liver disease including liver transplant patients. For instance, our team's work has shown that comparing term and pre-term children, both preterm infants (less than 37 weeks of gestation) and early term (37-38+6 wks gestation) infants have poorer neurodevelopmental outcomes compared to term (>39 wks) infants. Further, home literacy activities by caregivers can improve the outcomes for these children.
EF refers to a set of skills (e.g. Focus, Planning, Memory) that is important to school success. These are better predictors of academic success than IQ alone. Studies in this area include factors that influence and predict EF such as why some children have better EF skills than others and what affects this. We also look at the best intervention methods to improve these skills in children including the use of computer based intervention programs to improve EF. For instance, our team members have identified among children with preschool ADHD, the factors that determine who will go on to have persistent childhood ADHD and who will instead improve as they grow older.
Studies in the area include published work on child temperament and its relation to maternal mental and emotional health as well as correlation to child sleep and feeding behaviours. Factors that impact resilience in families of children with disability and interventions to improve parenting skills and reduce parenting stress are also part of ongoing research projects.
Our team researches on understanding the role of epigenetics and its mechanism by which the environment influences genetic expression for obesity susceptibility. The societal and economic impact of understanding this common clinical condition is significant.
We are involved in recruitment of subjects for the following projects:
The mission of the Division of Paediatric Haematology-Oncology is to provide quality clinical care for children with cancer and haematological disorders, and to develop effective therapies through clinical trials, improvements in haematological progenitor cell transplantation, translational research and international outreach.
We seek to improve the outcome of children with cancer in Southeast Asia through the integration of education, research and clinical care. The annual St Jude-Viva Forum in Paediatrics Oncology is the combined effort of the St Jude International Outreach Program and Viva Foundation for Children with Cancer, with the aim of providing paediatric oncologist in Asia the opportunity to learn and network with each other to foster collaborations. The Viva-Asia Acute Leukaemia and the Viva-Asia BMT Working Groups meet at the Forum each year to enhance regional collaboration and provide the opportunity to learn from each other's experience.
We have established a web conferencing system used for:
This is the successor trial of the recently reported Ma-Spore ALL 2003 study (Yeoh et al. J Clin Oncol 2012) for children and adolescents with Acute Lymphoblastic Leukaemia (ALL). It is a multi-centre study involving NUH and KK Women and Children's Hospital in Singapore, and the University Malaya and Sime Darby Medical Centre in Malaysia. The Malaysia Singapore (Ma-Spore) ALL 2010 study uses minimal residual disease (MRD) levels to determine early response to remission induction and tailor the intensity of chemotherapy
This multi-centre study for children and adolescents with AML involves the same institutions participating to Ma-Spore ALL 2010. It uses a modification of the MRC AML 10 treatment back bone replacing the second anthracycline block with high dose cytarabine in efforts to avert cardiotoxicity. It relies on MRD quantitation by flow cytometry and newly identified markers to assess response and select treatment intensity.
This study uses multi-agent chemotherapy, cisplatin, doxorubicin, high dose methotrexate, ifosfamide with etoposide for metastatic patients, and limb salvage surgery with biological materials (host bone) or prosthesis.
This study uses super-selective intra-arterial (ophthalmic artery) chemotherapy in the treatment of advanced intra-ocular retinoblastoma, Reese Ellsworth (RE) IV & V or International Intra-ocular Retinoblastoma Classification (ICIR) C, D & E.
Development of successive novel preparative regimens with aims to:
Development of laboratory approaches to study immune recovery of patients who received any of these:
MRD-based intervention in patients eligible for advanced cellular therapy and/or BMT, aimed to improve pre-BMT disease status and/or post-BMT disease control.
Philanthropic supported clinical research programmes in:
We have been a research centre with the "National Marrow Donor Program" (NMDP), USA and the "Centre for International Blood and Marrow Transplant Research" (CIBMTR), USA since 2002.
Mononuclear cells from bone marrow of patients on the Ma-Spore ALL 2010 and AML 2010 studies are interrogated using whole genome gene expression profiling and newly discovered algorithms to determine its value in disease subgrouping and prognosis.
Relying on extensive expertise in MRD monitoring and advanced instrumentation, we are validating recently discovered markers for MRD studies by flow cytometry in ALL and AML in both paediatric and adult patients.
We are studying the impact of gene mutations and pharmacogenetics on treatment outcome in children with ALL. Our study incorporates prognostically important mutations in genes like Ikaros, BIM, ABCB1 and IL15 mutations. We are also determining the pharmacogenomics of busulfan in Asian patients. Finally, we are studying Thiopurine Methyltransferase (TPMT) and Catechol-O-methyltransferase (COMT) polymorphisms in children with solid tumors who received cisplatin correlating the findings with those of audiometric testing.
We have developed unique methods to expand and activate NK cells, as well as to redirect NK cells and T lymphocytes through genetic engineering. These methods, which have been adapted to clinical-grade conditions, are being applied to treat children and adults with different malignancies. Research in the laboratory aims at discovering novel ways to redirect immune cells and at enhancing their anti-cancer potential.
Three cell therapy-based clinical protocols have been approved by NUH and Singapore General Hospital ethical boards and the Health Science Authority of Singapore. These protocols use expanded allogeneic NK cells to treat children and adults with various malignancies including AML, myelodysplastic syndrome, T-cell ALL, sarcoma and neuroblastoma.
We also organise a Pre-Forum Workshop specifically for our colleagues in the developing countries of the region. In the past years, we have discussed effective leukaemia and solid tumor protocols for countries with limited resources, palliative care, hospital registries, supportive care and effective fund raising.
In collaboration with St. Jude Children's Research Hospital, we have established a twinning partnership with the Southern Philippines Medical Centre (SPMC), a government referral centre for the whole island of Mindanao. We have also developed a Retinoblastoma Program for Mindanao Island in collaboration with Dana Farber Children's Hospital Cancer Centre and St. Jude Children's Research Hospital, supported by the Khoo Teck Puat Foundation and the Viva Foundation for Children with Cancer. The project aims at facilitating early diagnosis through education and developing SPMC as a referral centre.
We perform clinical as well as laboratory research so as to improve our understanding of kidney diseases in Asia with the ultimate aim of improving diagnostic and treatment approaches which may be unique to our population. Our group has published more than 100 papers in medical journals, presented more than 300 papers in medical conferences, and won more than 20 regional and international research awards. We have an established Paediatric Renal Immunology and Genetics Laboratory focusing on the genetics and immunology of kidney diseases in Asian children and adolescents.
Our genetics research arm involves genetic testing for patients with primary glomerular disease using advanced techniques, in collaboration with scientists from Agency for Science, Technology and Research (A*STAR) and NUS. Our preliminary research work have found that the genetics of our Asian population differ significantly from those of Caucasian patients. Genetic testing can potentially help doctors decide on individualised medical treatment.
Our immunology research arm is focused on nephrotic syndrome, mainly minimal change nephrotic syndrome and focal segmental glomerulosclerosis. The mechanisms of these diseases are still not fully understood. Our group was the first to describe the role of interleukin-13 (or IL-13) in children with relapses of the nephrotic syndrome. We have also developed an IL-13 overexpression animal model of nephrotic syndrome in order to further study the molecular signalling mechanisms that may be involved in the development of disease. Our latest work has identified the immunological abnormality in children with a serious form of nephrotic syndrome, namely focal segmental glomerulosclerosis, which predicts response to treatment with an anti-B lymphocyte monoclonal antibody, thus enabling children to be better treated to prevent progression to kidney failure. This work may in future lead to new potential targeted therapy in this disease.
Our clinical research arm focuses on the diagnosis and prevention of early cardiovascular disease in children with chronic kidney disease via means of novel biomarkers, ambulatory blood pressure monitoring, and specialised scans of the heart and blood vessels.
DRAGoN is a research consortium set up by paediatric nephrologists in NUS, in collaboration with scientists from the Genome Institute of Singapore, to investigate glomerular and proteinuric renal diseases in Asians.
Primary glomerular disease, including focal segmental glomerulosclerosis (FSGS), is a common cause of renal failure in adults and children worldwide. Current treatment is empirical and involves immunosuppressive drugs which are toxic, expensive and with only moderate response rates. Proteinuric or familial haematuric diseases are traditionally monogenic diseases, involving as many as 40 to 50 known genes. With new sequencing techniques, concomitant variants in different genes are increasingly recognised as important. In addition, extrapolating from studies in African Americans involving MYH9 and APOL1 as significant risk alleles, FSGS can also be a polygenic disorder.
Genetics of renal kidney diseases in Asia is largely understudied. There has been evidence that the genetic causes of glomerular diseases in Asia differ from other parts of the world. For example, Asian children with sporadic steroid-resistant nephrotic syndrome have a much lower prevalence of podocin mutations compared to Caucasian and Middle East patients. A dedicated Asian genetic network allows for more comprehensive and systematic research to decipher the unique Asian genetic makeup. Additionally, Malays who have a higher risk of renal failure are largely understudied in the world.
Our mission is to advance our understanding of the demographics, causes and genetics of glomerular diseases in Asians, and eventually to provide an evidence base for clinical management and explore innovative treatment strategies for genetically based diseases. We hope this network can systematically study the genetics of renal diseases in Asia.
This study is divided into two parts, namely Genome-wide association studies (GWAS) and Next Generation Sequencing (NGS).
This aims to identify genetic risk alleles that may be important in disease susceptibility in primary sporadic FSGS in Asia.
This aims to identify potentially disease-causing genetic mutations in patients with primary proteinuric glomerular diseases which are either familial; of congenital or infantile-onset; or resistant to all forms of immunosuppressive therapy.
We will first screen >100 genes known to cause glomerular diseases using targeted gene sequencing. If no definitely or probably pathogenic variants were found in known genes, then exome sequencing may be performed to identify novel genes.
Our secondary aims include preliminary in-vitro functional work on the identified genetic variants, and establishment of a comprehensive phenotype database in Asia to foster future collaborations.
There is no age limit for recruited subjects. Adult subjects are welcome.
Availability of histological evidence is important. Primary evidence should be as one of the following forms:
Only a single collection of blood or saliva is required and this can be performed during visits to their doctors. Saliva will be collected either by asking the subject to spit into a cup, or by using absorbent sponges.
By possibly identifying genetic causes of the disease, it may lead to more directed treatment strategies, which may translate to decreased side effects and lower healthcare costs. Identification of risk alleles for the FSGS in Asia may allow estimation of lifetime risks for kidney disease, as well as possibility of response to therapy and risk of disease progression.
Apart from the standard risks involved in blood-taking, there are no additional risks involved.
We welcome collaborators from healthcare institutions in Asia. If you are a clinician or researcher and would like to find out more about this study, please email Dr Ng Kar Hui at
email@example.com for more details.
We cover shipping costs, and provide co-authorship in all publications.
Level 12 NUHS Tower Block, 1E Kent Ridge Road, Singapore 119228
Department of Paediatrics
Tahir Foundation Building, MD1 Level 15, 12 Science Drive 2, Singapore 117549
Our research focuses on understanding the molecular basis of human genetics disorders in children. The Human Genetics Laboratory (HUMGEN Lab) was established in 1989 to develop molecular diagnostic methods for single gene disorders such as Duchenne muscular dystrophy (DMD), retinoblastoma (RB), spinal muscular atrophy (SMA), haemophilia and glucose-6-phosphate dehydrogenase (G6PD) deficiency. This subsequently expanded to cover other disorders such as Alagille syndrome, Wilson's disease, Hirschsprung's disease, Wilm's tumor, neurofibromatosis, myotonic dystrophy, charcot-marie tooth disease, limb girdle muscular dystrophies, Marfan's syndrome and Tarui's disease. Mutation screening of rare disorders can contribute towards early detection and improved clinical management in patients.
As part of the clinical interface with research, the lab had developed genetic tests for applications in molecular analysis, prenatal diagnosis and genetic counselling of various congenital disorders in Singapore. We have been actively engaged in the areas of molecular diagnostics and therapeutics, as well as in providing an understanding of the mechanisms underlying normal and disease states in subjects. Our current interest is in the application of techniques and strategies in studying disease susceptibility and elucidating the molecular architecture of genetic traits in specific conditions. We also conduct multidisciplinary collaborations with other departments and institutions to infuse innovative insights into our research programme.
The ultimate goals of our research are to provide clinically relevant outcomes in the form of:
Ongoing research interests of our group include:
Our group is interested in leveraging on genetic and genomic knowledge and technological advances to improve the diagnosis and prevention of heritable disorders, in the process developing novel and innovative molecular tests using technology platforms.
Translational research is focused on developing novel and innovative DNA-based diagnostics for application to preimplantation genetic testing (PGT), prenatal diagnosis (PND) and postnatal genetic testing and screening of inherited disorders that are common (alpha-thalassemia, beta-thalassemia, spinal muscular atrophy), rare (Prader-Willi and Angelman syndromes), or diagnostically challenging (fragile X-related and other trinucleotide repeat disorders). We aim to develop elegant solutions to practical barriers that hinder widespread adoption of genetic testing and screening for these disorders.
Consistent with this goal, our group has developed a molecular toolbox for the analysis of the fragile X-related disorders, which includes a novel single-step screening assay for rapid high throughput identification of affected and at-risk individuals, a fluorescent PCR assay capable of accurately sizing all normal and premutation FMR1 alleles and detecting all full mutation alleles, and a single-tube assay for detecting and sizing all unmethylated and methylated normal and premutation FMR1 alleles and determining methylation status of full mutation alleles. Each assay can be performed at a fraction of existing screening and diagnostic test costs.
A USPTO patent has been awarded for this screening concept, and the FMR1 molecular analysis toolkit has been licensed and is now available commercially. The innovative testing and screening strategy employed here is potentially applicable to other disorders caused by expansion of simple sequence repeats within a gene. Similar screening and testing assays for other trinucleotide repeat disorders based on the same strategy used in the FMR1 molecular analysis toolkit are being developed gradually. We will also be exploring the use of third generation sequencing platforms for accurate sizing of trinucleotide repeat hyperexpansions in affected individuals.
Our efforts have also been directed towards the simplification of preimplantation genetic testing of monogenic disorders (PGT-M). Generally in PGT-M, considerable assay customisation is required for each couple's specific gene mutation(s), and for identifying informative linked markers, even if the disease gene is the same. Linked markers serve an important role to independently corroborate mutation genotype results, which are vulnerable to misdiagnosis from allele dropout or exogenous DNA contamination. In situations where the mutation(s) is/are unknown or cannot be genotyped, or where an at-risk parent does not wish to know his/her mutation status, linked markers provide the only means for PGT-M of that disorder.
To address these issues, we have been gradually developing panels of highly multiplexed microsatellite markers adjacent to various disease genes, starting with the most common inherited disorders. We have developed a panel of 11 STR/STS (short tandem repeats / sequence tagged site) markers within and flanking the alpha-globin gene cluster, for PGT-M of all common deletional determinants of alpha-thalassemia. This single-tube assay tests for all common alpha-globin gene deletions and simultaneously generates multi-marker diplotypes for independent corroboration of the deletional status of embryos. The high heterozygosity of every STR marker in the panel ensures sufficient panel informativity for the assay to be used in virtually all alpha-thalassemia PGT-M cases without modification. Similar multi-marker panels flanking other common disease gene loci have been developed, including those for beta-thalassemia, haemophilia A, and trinucleotide repeat disorders such as fragile X syndrome, myotonic dystrophy type 1, and Huntington disease.
We are now expanding our translational research into PGT for whole chromosome aneuploidy (PGT-A) and other structural rearrangements (PGT-SR), both of which can be performed using next-generation sequencing (NGS) platforms. Although performing PGT-M for an inherited disease simultaneously with PGT-A to screen for chromosome aneuploidy is possible, the few reported strategies are inefficient, one contributing factor being the incompatibility of whole genome amplification methods required for PGT-M and PGT-A. Furthermore, while low-coverage NGS methods are sufficient to detect aneuploidy clearly most of the time, a significant fraction of ambiguous results of unknown cause are observed. Rather than a low-coverage shotgun sequencing of random fragments, massively-multiplexed targeted SNP amplicon sequencing may produce more consistent chromosome copy number in PGT-A. Such a strategy may also be equally applicable to PGT-SR of reciprocal and Robertsonian translocations. Finally, this PGT-A strategy can be easily combined with mutation-carrying amplicons for simultaneous PGT-M for inherited diseases.
In the course of our diagnostic development work, we have also made interesting observations that have led to identification of novel gene rearrangements at the alpha-globin gene cluster, as well as nucleotide variants within trinucleotide repeats. These findings are important as they may have significant implications for diagnostic test accuracy and correct results interpretation.
Our group's current basic research efforts have been focused on elucidating the function of genes implicated in developmental disorders, in particular oral clefting. Oral clefts rank among the most common congenital malformations globally, and represent a major public health burden especially in Third World countries. The etiology of oral clefts is complex, with strong evidence that multiple genes control risk to oral clefts, some of which may interact with common environmental risk factors such as maternal smoking, alcohol consumption, and vitamin supplementation. As part of an international collaborative group aiming to identify genetic factors predisposing to idiopathic oral clefting, several genes that increase risk for oral clefting were identified, either directly or through interaction with environmental factors.
We have utilised the zebrafish Danio rerio, an established vertebrate model, to study the effects of gene function and dysfunction on normal and abnormal development. In particular, we examined an interesting maternal zygotic transcription factor Interferon Regulatory Factor 6 (IRF6), which appears to activate different genes at different stages of embryonic and fetal development. We have been focusing on its function in the zygote and early embryo. Deletion of its protein interaction domain, but not its DNA binding domain, leads to early developmental arrest at gastrulation followed by embryo rupture at the animal pole. Using transcriptome analysis of such perturbed embryos, we identified two very highly down-regulated direct target genes of this transcription factor. We have shown that they are directly bound and activated by IRF6. Future work will involve characterisation of other highly dysregulated genes identified from the microarray analysis.
Our research focuses on many different aspects of neonatology, as our work involve both well infants and sick premature infants.
For well infants, we are experienced in developing the cord thyroid-stimulating hormone (TSH) screening for detecting congenital hypothyroidism. It is now translated from bench to clinical care nationwide. We collaborated with the Audiology Department in development of Universal Newborn Hearing Screening and establishing protocols for various at-risk groups. We also worked with the National Expanded Newborn Screening committee in developing national norms for metabolic screening with tandem mass spectrometry. We continue to research novel screening technologies to improve the health of newborns through early screening.
Working with collaborators, we had also performed studies looking at common neonatal diseases like neonatal jaundice and its genetic predisposition as well as early detection and treatment.
In the field of maternal and neonatal medicine, we had won grants studying the effect of maternal chronic hepatitis B infections on immunoprophylaxis failure of the hepatitis B vaccination programme.
For premature infants, we had won grants studying the clinical applicability of cranial and vascular ultrasonography. We also have animal studies in the field of neonatal haemodynamics.
In collaboration with Duke University, we completed a pilot trial on the safety and feasibility of autologous cord blood cell infusion for term infants with hypoxic-ischemic encephalopathy in Singapore.
The ultimate aim of our research is to translate basic research into further understanding of the common neonatal diseases and developing better care for our fragile patients.
Ongoing research of our group includes: