Theory and Practical training in Embryology (Basic ART course)

1. Aseptic Techniques
2. Set up for cases
   • Preparation of sperm and oocyte wash dishes
   • Oocyte culture dishes
   • Medium for sperm preparation
3. Oocyte retrieval
• Oocyte identification and maturation
• Oocyte grading
4. Sperm Preparatoin for IVF and ICSI
• Swim Up technique
• Density Gradient
• Sperm washing procedures for Normozoospermic, Oligozoospermic severe, Oligoteratozoospermic sperm samples.
5. Insemination procedures
   • ICSI and IVF
   • Long Insemination
   • Brief Insemination
   • Removal of cumulus
6. Dish Preparation for embryo culture
• Microdroplet culture
7. Fertilization Check
• 2PN Identification
8. Embryo grading and culture
9. Embryo Selection for embryo replacement on Day-3, Day-4, Day-5
• Dish Preparation
• Transfer of selected embryos into the dish
10. Embryo Replacement
• Usage of catheters
• Loading of embryos
11. Embryo Cryopreservation
• Cleavage Stage
• Blastocyst Stage
• Cryopreservation Medium preparation
• Loading, freezing, seeding, storage
12. Embryo Thawing
• Cleavage Stage
• Blastocyst Stage
• Thawing medium preparation
13. Record Keeping
14. Maintenance of the Laboratory
15. Quality Control
    

Details
Course Duration: 1 month (excluding Sundays)
Course Fee: $ 12,000/-
Venue of training: IVF lab DSOT, NUH

Applicants for the course will be selected based on their experience and qualification. Applicants should have at least a BSc (Hons) in Biological Sciences, Microbiology, etc with an adequate knowledge of embryology.

All trainees will receive a certificate upon completion of the course.

Interested applicants, please write to Ms Joyce Mathew.

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